Figure 4From: Exploring smooth muscle phenotype and function in a bioreactor model of abdominal aortic aneurysmHuman SMC morphology. Cells were explanted from human aneurysmal wall tissue (AAA), or human saphenous vein (SV) and maintained in culture in full growth medium. (A) Immunocytochemical staining of α-SMA (green) and SM-MHC (red) and co-localisation in human AAA SMC. Magnification ×400, scale bar = 100 μm. (B) Representative phase contrast images of aortic SMC, (C) SV-SMC and (D) human AAA-SMC, scale bar = 100 μm. (E) The mean cell areas of 100 individual cells per patient were quantified using Image J. All n = 12, ***P < 0.001.Back to article page