Figure 1
Measurement of serum levels of VEGF in the TNBS and DSS models of UC. The level of total serum VEGF-A isoforms was measured by ELISA in serum from control, vehicle, and TNBS treated mice (A); n = 4. The level of total serum VEGF-A isoforms was measured by ELISA in serum from control and DSS treated mice (B); n = 4. Serum protein was measured at either the acute (C) or chronic stages of the model (D); n = 5. Levels of total serum VEGF-A isoforms was measured in serum of animals treated with Ad5-CMV-rVEGF164b at 7 days after injection of Ad5-CMV-rVEGF164b using doses of 0 (control), 1 × 107, 1 × 109, and 1 × 1011 viral particles (E). An estimate of the persistence of expression of rVEGF164b was generated by measuring total serum VEGF-A isoforms in the serum of animals injected with 1 × 1011 viral particles of Ad5-CMV-rVEGF164b at days 0, 10, and 21 (F).