Figure 4

UCX cells induce T-cell conversion into Treg cells. FACS-sorted CD3+ CD4+ CD25- T-cells were activated with anti-CD3, anti-CD28 and IL-2 alone (A) and with the addition of TGF-β (B). The effect of irradiated UCX® cells (C) and BM-MSCs (D) in T-cell conversion was analyzed in cultures without exogenous TGF-β by flow cytometry analysis of CD25 and Foxp3 expression.