Figure 5

Detection and MACS enrichment of survivin specific CD8+ T cells. CD8+ T cells from donor 2, were stimulated three times with autologous DC electroporated with mRNA encoding full length survivin and IL12. The fourth stimulation was performed with mRNA encoding Ubi-survivin and IL12. The T cells were stained with survivin tetramers. Each symbol represents a separate mini-culture. After four stimulations the T cells were MACS sorted en stimulated with feeder-mix. Depicted in the middle panel is the percentage of survivin specific T cells of one representative well after fourth stimulation, just before MACS sorting. In the right hand panel the percentage of MACS sorted survivin specific T cells is depicted after one week on feeder mix. A) panels left, middle and right show the data obtained with the survivin 5 epitope. B) panels left, middle and right show the data obtained with the survivin 96 epitope.