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Figure 1 | Journal of Translational Medicine

Figure 1

From: Functional hyper-IL-6 from vaccinia virus-colonized tumors triggers platelet formation and helps to alleviate toxicity of mitomycin C enhanced virus therapy

Figure 1

Vaccinia virus strains and recombinant protein expression. (A) Constructs of the recombinant vaccinia virus strains GLV-1h68 and GLV-1h90 which were derived from the wild-type Lister strain. The viral genes encoding for F14.5L, thymidine kinase (TK) and hemaglutinin (HA) were interrupted by the foreign gene expression cassettes encoding for a Renilla-luciferase-GFP (Ruc-GFP) fusion protein, beta-galactosidase (lacZ), beta-glucuronidase (gusA) and hyper-IL-6, respectively. (B) Infection of DU-145 cells led to expression of the marker genes as visualized by Western blot analysis of cell lysates and supernatants of infected cells at 2, 4, 8, 12, 24, 48, and 72 hpi. Mock-infected cells (-) served as negative control. (C) Quantitative (ELISA) measurements of (hyper-) IL-6 in cell lysates and supernatants of GLV-1h90-infected DU-145 cells.

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Journal of Translational Medicine

ISSN: 1479-5876

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