Figure 5

The effects of KRAS knockdown on ERK phosphorylation following CXCL12 treatment. Pancreatic cancer cells, FG and PANC-1 (left and right immunoblots respectively) were transfected with control or KRAS siRNA (100 nM). Cells were serum-starved and then exposed to CXCL12 (100 ng/ml) or EGF (100 ng/ml) for 15 min. Immunoblotting was performed for K-Ras and phospho-ERK1/2. Total ERK1/2 and GAPDH were used as loading controls.