Figure 5
Time course changes in L-[U-14C]-phenylalanine incorporation into proteins. Confluent L6 cells were cultured in medium containing very low amounts of amino acids (0,14 mM) for 24 hours, and then refed in low (0,28 mM) or high (9 mM) concentration of total AA concentrations, with constant specific radioactivity of phenylalanine (40 μCi/μmol phe). Cells were harvested by TCA-precipitation of proteins. (* p< 0.01 vs. low AA).