Adoptive cell therapy with autologous tumor infiltrating lymphocytes and low-dose Interleukin-2 in metastatic melanoma patients

Table 4 T-cell responses detected in TIL infusion products

a) IFN-γ ELIspot analyses
Patient ID		1	2	3	6	7	11
HLA-type		A2	A2/A3	A3, A11	A2	A2	A1, A3
A2	Bcl-2_WLD	NR	43	-	NR	301	-
	Bcl-x_RIA	NR	165	-	NR	197	-
	hTERT_ILA	180	456	-	363	462	-
	CB9L2_ALY	232	135	-	293	441	-
	CB9 204_ILI	488	502	-	325	408	-
	NY-ESO 1_SLL	180	564	-	158	279	-
	MAGE A1_KVL	NR	57	-	NR	190	-
	MAGE A3_FLW	91	67	-	118	250	-
	SUR1M2_LML	ND	96	-	ND	349	-
	SUR9_ELT	ND	62	-	ND	274	-
	MART-1_ELA	ND	324*	-	193*	NR	-
	gp100_ITD	ND	106	-	ND	ND	-
A3	Bcl-x_RIA	-	284	111	-	-	59
	SUR18K10_RIS	-	ND	112	-	-	NR
	SUR53_DLA	-	96	ND	-	-	82
	Rho C (nat)_RAG	-	ND	114	-	-	NR
	Rho C (mod)_RLG	-	ND	103	-	-	NR
	TAG_RLS	-	ND	ND	-	-	42*
Number of responses/total screened		5/15	14/24	4/9	6/15	9/15	3/13
Background		99	46	86	130^a/92^b	131	29
b) MHC multimer staining
Patient ID		1	2 ^c	3	6	7	11 ^d
HLA-type		A2	A2, A3, B7	A3, A11	A2	A2	A1, A3
A2	MART-1_ELA	0.013	0.07*	-	1.10*	0.008	-
	gp100_YLE	0.010	NR	-	10.0	NR	-
	gp100_KTW	0.010	NR	-		NR	-
	gp100_ITD	0.002	NR	-	0.018	NR	-
	GnT-V_VLP-9	0.015	NR	-	NR	NR	-
A3	TAG_RLS	-	NR	NR	-	-	0.60*
Number of responses/total screened		5/146	1/162	0/14	3/146	1/146	1/21

a) IFN-γ ELIspot analyses; Responses are defined as number of spots per 1 x 10⁵ T cells, except for patient 7 (2.5 x 10⁴ cells) and patient 11 (1.25 x 10⁴ cells). Background level shown at the bottom of the table has been subtracted in the shown responses. ^a/b: two different assays have been performed wherefore two background values are present.
b) MHC multimer staining; Antigen-specific T cells are given in percentage of CD8⁺ T cells. Examples of MHC multimer stainings can be seen in Additional file 3. ^cThe TILs analyzed were frozen two days after infusion. ^dThe TILs analyzed were frozen one day after infusion.
Last row indicates the number of detected responses out of the total number of peptides screened. NR: no reactivity, ND: reactivity testing not done, -: not tested due to HLA-discrepancy.
*Indicating responses found in both methods. nat: natural, mod: modified.

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