Figure 4

mRNA and protein expressions of skeletal muscle damage markers in ischemic quadriceps by day 21 following CLI procedure (n = 8). A and B) showing serum and ischemic muscle microRNA-206 levels, respectively. For (A) and (B),* vs. other groups with different symbols, p < 0.0001. C) mRNA expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α).* vs. other groups with different symbols, p < 0.0001. D) mitochondrial cytochrome C (Cyt C) protein expression.* vs. other groups with different symbols, p < 0.008. E) Cytosolic cytochrome C protein expression.* vs. other groups with different symbols, p < 0.004. Statistical analysis in each group by ANOVA followed by Bonferroni multiple comparison post hoc test. CLI = critical limb ischemia; Cil = cilostazol; Clo = clopidogrel.