Figure 4
Effect of neoadjuvant immuno-gene therapy on distant tumor growth. (A) Mice were inoculated with tumor cells on one flank and 5 days later on the opposite flank. The perpendicular tumor diameter was measured every 5 days, and tumor volume (V) was calculated by the formula for a rotational ellipsoid: V (mm3) = length × width2/2. When the opposite tumor foci reached 50 mm3, the larger tumor was injected with PBS, 5 × 108 pfu Adel55, Adel55-cHSF1, Adel55-HSF1i, Adel55-HSP70 or Adel55-HSP90 and surgically excised 72 h post injection. The progression of the opposite tumor foci was measured for one month. (A) C57B/6 mice inoculated with B16 tumor cells (n = 8). (B) Balb/C mice inoculated with CT26 tumor cells (n = 8). *represent statistical significance (P < 0.05, Adel55-cHSF1 vs. Adel55-HSP70 or Adel55-HSP90). (C) The picture shows the opposite tumor foci of different groups of Balb/C or C57B/6 mice bearing B16 or CT26 tumors and tumors dissected at day 30.