Figure 5

BCG and IFN-γ matured DC induce primary CD4⁺ Th1-type T cell responses as opposed to BCG matured or immature DC PBMC were stimulated with KLH-loaded DC matured with either BCG alone (1.6 × 10⁶ CFU/ml) or BCG and IFN-γ (1000 U/ml). Every 10–14 days the cell lines were re-stimulated with the same DC used in the initial in vitro stimulation. After 3–5 rounds of such stimulation, the resulting cells were analyzed for KLH-specific proliferation in a ³H-thymidine incorporation assay as described in Materials and Methods (A). The KLH-specific T cells were non-specifically stimulated with anti-CD3 and PMA for 8 h, including GolgiPlug™ for last 6 hours. Subsequently the cells were stained for intracellular IFN-γ, IL-4 or IL-2 (B). The data are from one representative experiment out of four similar experiments.