Figure 2
IFN-γ treatment in combination with BCG induces TNF-α in a higher percentage of dendritic cells than treatment with BCG alone DC were treated with 1.6 × 106 CFU/ml of BCG, with or without 1000 U/ml IFN-γ, or left untreated (immature). Twenty hours later GolgiPlug™ was added to inhibit cytokine secretion and the cells were cultured for an additional 16 h prior to intracellular staining with anti-TNF-α-FITC and extracellular staining with anti-HLA-DR, DP, DQ-PerCP. Data shown here are from a representative experiment out of four similar experiments.