Figure 1

HLA-A Locus, Exon II nucleotide sequence alignment of alleles relevant to the discussion of this manuscript. The consensus sequence is shown in green in areas containing polymorphic sites and in yellow at conserved areas (k oligos). Genomic regions covered by variant oligos are indicated by boxes with colored borders (red: HLA-A*02; violet: sub-types of HLA-A*02 different from A*0201; green: HLA-A*0301, purple: HLA-A*2301; blue: HLA-A*29) corresponding to the sequences shown in Figure 2. Dashed borders indicate overlapping oligos. Dotted borders indicate variant oligos 1-SP-A-02a and b that contain an additional G > A switch in a peripheral region to test the influence of this mismatch in various conditions. In black are SNP in areas for which no variant alleles were designed ("unknown SNP"). The dashed line represents the areas where most "unknown SNP" are present for HLA-A*02 and A*29 (blue) or only for HLA-A*02 (orange).