Fig. 6

AP203 exerts potent antitumor activity in two humanized mouse models. A Anti-tumor efficacy of AP203 in the immunodeficient SCID/Beige mice xenografting with BxPC-3 pancreatic tumors reconstituted with human immune cells. Mice bearing BxPC-3 xenograft tumors were injected intraperitoneally with PBS control (10 mg/kg) or AP203 (0.13, 1.3, and 13 mg/kg) or its parental antibodies (each 10 mg/kg) in combination for twice a week. The tumor volumes were measured and recorded twice a week and presented as mean ± SEM. Differences were found to be statistically significant at * < 0.05 and *** < 0.001. B Analysis of infiltrating human lymphocyte population in BxPC-3 tumor tissues. At day 35, BxPC-3 tumor tissues were collected for analysis of the tumor-infiltrating human lymphocytes by flow cytometry. C Anti-tumor efficacy of AP203 in PD-1/PD-L1/CD137 triple knock-in mice bearing MC38 colon cancer cells. MC38 tumor-bearing mice were injected intraperitoneally with PBS control or AP203 or its parental antibodies in combination for twice a week. The tumor volumes were measured and recorded twice a week and presented as mean ± SEM. Differences were found to be statistically significant at ** < 0.01. D Altered TILs populations in MC38 subcutaneous colon tumors following treatment with bispecific antibody AP203. Flow cytometry analysis was used to determine and quantify different immune cells (% of either live or CD45 + cells)