Fig. 4

Comparison of AP203 and its parental antibody in T cell activation, enhanced memory recall response and overcoming Treg-mediated immunosuppression. A Effect of AP203 in the activation of primary CD4 + T cells in the allogeneic MLR. ELISA was used to measure the secretion of IL-2 and IFN-γ from three healthy donors (DP). AP203 showed superior stimulatory activity in CD4 + T cell activation compared to parental antibody alone or in combination. B Memory T cell recall assay measuring IL-2 and IFN-γ produced by human CD4 + memory T cells and CD8 + memory T cells, respectively. The secretion of IL-2 and IFN-γ in the culture supernatant was measured by ELISA. AP203 exerted superior CD4 + and CD8 + memory T cell recall response than its parental antibody alone or in combination. C Effect of AP203 in overcoming Treg-mediated suppression of effector T cell proliferation and IL-2 production by CD4 + T cells in the allogeneic MLR. AP203 exhibited superior bioactivity in overcoming Treg-mediated immunosuppression than its parental antibody alone or in combination