Fig. 2

Screening of potent CD137 binders through OmniMab phagemid library. A Top four high-affinity binders that bind to CD137. The binding affinity of four CD137 antibody candidates to recombinant CD137-ECD-Fc was determined using ELISA. Clone #7 and clone #54 showed the best binding affinity to CD137-ECD-Fc B Agonism analysis of the top four CD137 antibody candidates using a CD137/NF-κB reporter assay. Clone #54 showed the stronger CD137 agonist activity. C Induction of IL-2 and IFN-γ secretion by the top four CD137 antibody candidates. The secretion of IL-2 and IFN-γ in the culture supernatant was measured by ELISA. Clone #54 exhibited the best biological activity in inducing secretion of the cytokines IL-2 and IFN-γ