Fig. 3

ROS regulation played a critical role in the preventive effect of ALT against OSCC cells. A–C Cell morphology observed by phase contrast microscope and cell viability analysis (scale bar = 100 µm). D–F The number of colonies assessed and quantified by crystal violet staining. G–J Flow cytometry-based Annexin V-FITC/PI labeling of apoptotic cells. We used 15 µM ALT to treat CAL27 cells and SCC9 cells. (**p < 0.01, ***p < 0.001, ****p < 0.0001 versus the Control group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 versus the ALT group)