Fig. 1

ALT inhibited cell viability and triggered OSCC cell apoptosis. A Chemical structure of ALT. B The inhibitory effects of ALT on PC-9, LOVO, U251, CAL27, and SCC9 cells. C, D Cell viability was measured in CAL27 and SCC9 cells after being treated with ALT for 24 h. E, F Cell viability was measured in CAL27 and SCC9 cells after being treated with ALT for indicated periods. G–I CAL27 and SCC9 cells were seeded into 6-well plates and then treated with various dosages of ALT as indicated. The number of colonies was assessed and quantified by crystal violet staining. J–M Flow cytometry analysis after Annexin V-FITC/PI double staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus the Control group)