Fig. 3

Administration of SDMA attenuates renal tubulointerstitial fibrosis in unilateral ischemia–reperfusion injury (UIRI) kidneys. Male c57 mice received UIRI operation for 35 min and were sacrificed at day 11. Renal injection of 50 μL of NS or 10 mM SDMA (25 µmol/kg) was performed during UIRI operation. Renal fibrosis was assessed by Masson’s trichrome staining (A) and quantified (B). Scale bar = 200 µm for upper figures and scale bar = 100 µm for lower figures. The expression of fibronectin, collagen-I, α-SMA and Snail were analyzed by Western blotting (C) and quantified (D). One representative of at least three independent experiments is shown. Data represent mean ± SD. ND represents not determined. *p < 0.05. **p < 0.01. ***p < 0.001