Proteomics and cytokine analyses distinguish myalgic encephalomyelitis/chronic fatigue syndrome cases from controls

Table 3 Effects of clinical data on EV, plasma cytokines and plasma proteomics according to robust linear regression

EV cytokines	Covariate-Interaction^a	β coefficient	P-value	Q-value
CXCL1	Age	− 0.013	0.001	0.035
CCL11	Age	0.032	0.001	0.035

Plasma cytokines	Covariate-Interaction^a	β coefficient	P-value	Q-value
Leptin	BMI	0.123	< 0.001	< 0.001
Leptin	Male	− 1.119	< 0.001	0.001
CSF2	BMI	0.123	< 0.001	< 0.001
CSF2	Male	− 1.230	< 0.001	< 0.001
CCL2	Intercept	0.690	0.486	0.900
	ME/CFS	− 2.696	0.046	0.886
	Age	− 0.037	0.006	0.152
	ME/CFS:Age	0.075	< 0.001	0.009
CSF3	Intercept	0.576	0.573	0.900
	ME/CFS	− 1.994	0.151	0.886
	Age	− 0.047	0.001	0.038
	ME/CFS:Age	0.075	0.001	0.009

Plasma proteomics	Covariate-Interaction^a	β coefficient	P-value	Q-value
SAA1	Age	0.047	< 0.001	0.049
PFN1	Intercept	0.809	0.322	0.996
	ME/CFS	− 0.996	0.417	0.914
	Male	0.081	0.795	0.967
	ME/CFS:Male	− 1.901	< 0.001	0.030
IGHA2	Intercept	0.454	0.561	0.996
	ME/CFS	− 2.048	0.084	0.907
	( +) IBS	− 2.811	0.001	0.137
	ME/CFS:( +) IBS	3.467	< 0.001	0.019
LRG1	Intercept	0.261	0.727	0.996
	ME/CFS	− 1.082	0.336	0.907
	( +) IBS	− 2.502	0.001	0.153
	ME/CFS:( +) IBS	3.093	< 0.001	0.026

Similarly, ME/CFS:Age or ME/CFS:( +) IBS indicate the interaction term between ME/CFS and age or subjects with IBS. ( +) IBS denotes subjects with IBS. Categorical variables were coded as follows: Cohort: control = 0; ME/CFS = 1; IBS: no IBS = 0; with IBS = 1
^aME/CFS:Male indicates the interaction term between ME/CFS and males

ISSN: 1479-5876