Fig. 4

The stress-injured epithelial Beas-2B cells facilitated M1-polarization of macrophages. A A co-culture system of Beas-2B cells treated with IR and/or BLM and PMA-pretreated U937 cells. B Flow cytometric analysis of the ratio of CD86 + (M1 marker) and CD163 + (M2 marker) macrophages within above co-cultured U937 cells. C-D The percentage C and mean fluorescence intensity (MFI) D of CD86 + cells in CD68 + population within the above co-cultured U937 cells. E–F The percentage E and MFI value F of CD163 + cells in CD68 + population within the above co-cultured U937 cells. *p < 0.05, **p < 0.01, ***p < 0.001