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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: P21 facilitates macrophage chemotaxis by promoting CCL7 in the lung epithelial cell lines treated with radiation and bleomycin

Fig. 3

P21 was overexpressed in the RILI, BIPF, and BIPF + RILI model mice. A Venn diagram illustrating the intersection of the upregulation genes in three groups of model mice: RILI, BIPF, BIPF + RILI (left), and the gene list and fold change of top 5 common upregulated genes (right). B–C RNA expression levels of top three genes from Venn diagram A in MLE-12 B and Beas-2B cells C after IR and/or BLM treatment. D–E Protein expression level of P21 in MLE-12 D and Beas-2B cells E. F Immunofluorescence images of P21 (red color) and DAPI (blue color) in Beas-2B cells. Scale bar = 20 μm. G The proportion of P21-positive Beas-2B cells after IR and/or BLM treatment. H Viability of Beas-2B cells after IR and/or BLM treatment. I Immunohistochemistry images of P21, pro-SPC, and F4/80 in the lung tissues of mice model RILI, BIPF, and BIPF + RILI at week 4 after IR. Scale bar = 50 μm. J Positive area of P21, pro-SPC, and F4/80 in the lung tissues of above model mice. *p < 0.05, **p < 0.01, ***p < 0.001

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