Fig. 9

IGF2BP3 promoted M1 macrophage polarization in OA. A Flow cytometry analysis of CD206 and CD86 expression of BMDMs after LPS treatment (positive control of M1), IL-4 treatment (positive control of M2), IGF2BP3 overexpression, and IGF2BP3 knock down (n = 3). B Immunoblotting of IGF2BP3 expression, polarization (iNOS, CD206), inflammation (IL-1β) and anti-inflammation (IL-4) genes in IGF2BP3 overexpressed BMDMs (n = 3). C Immunoblotting of IGF2BP3 expression, polarization (iNOS, CD206), inflammation (IL-1β) and anti-inflammation (IL-4) genes in IGF2BP3 knock down BMDMs (n = 3). D Cell fluorescence of polarization (iNOS, CD206), inflammation (IL-1β) and anti-inflammation (IL-4) genes in IGF2BP3 overexpressed BMDMs (n = 3), Scale bars: 25 μm. E Cell fluorescence of polarization (iNOS, CD206), inflammation (IL-1β) and anti-inflammation (IL-4) genes in IGF2BP3 knock down BMDMs (n = 3), Scale bars: 25 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant