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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X

Fig. 7

Effect of salubrinal, guanabenz and empagliflozin on STIM1 and ORA1 expression in LMNA R321X-cardiomyocytes. A Upper panel: Representative Western blotting of STIM1 ad ORA1 performed on lysates from LMNA R321X-cardiomyocytes treated with either 200 µM salubrinal or 20 µM guanabenz for 48 h and from LMNA WT-cardiomyocytes as control. The triplicate of each experimental conditions in the representative western blotting corresponds to three different biological replicas. Lower panel: densitometric analysis of the immunoreactive bands corresponding to STIM1 and ORA1, in the experimental conditions shown in the upper panel, normalized for total protein content and reported as % of the control condition (WT). Data are represented as mean ± SEM. Statistical analysis was performed on three independent experiments and significance calculated by one-way ANOVA and Tukey's multiple comparisons test (°°°p = 0.0001, for WT vs R321X; ***p = 0.0003, ****p = 0.0001 for untreated R321X vs treated R321X). B Upper panel: Representative Western blotting of STIM1 ad ORA1 performed on lysates from LMNA R321X-cardiomyocytes treated with either 10 µM empagliflozin for 48 h and from LMNA WT-cardiomyocytes as control. The triplicate of each experimental condition in the representative western blotting corresponds to three different biological replicas. Lower panel: densitometric analysis of the immunoreactive bands corresponding to STIM1 and ORA1, in the experimental conditions shown in the upper panel, normalized for total protein content and reported as % of the control condition (WT). Data are represented as mean ± SEM. Statistical analysis was performed on three independent experiments and significance calculated by one-way ANOVA and Tukey's multiple comparisons test (°°°p = 0.0001, for WT vs R321X; ***p = 0.0003, for untreated R321X vs treated R321X)

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