Fig. 3

Effect of salubrinal and guanabenz on the expression of markers of PERK and AKT pathways in LMNA R321X-cardiomyocytes. A Representative Western blotting of phospho-eIF2α (p-eIF2α), ATF4, CHOP, full length PARP (PARP-FL) and cleaved PARP (PARP-CL), p-PERK and p-AKT performed on lysates from LMNA R321X-cardiomyocytes treated with either 200 µM salubrinal or 20 µM guanabenz for 48 h and from LMNA WT-cardiomyocytes as control. The expression of Na⁺/K⁺-ATPase α subunit was also checked in all samples as control. The triplicate of each experimental condition in the representative western blotting corresponds to three different biological replicas. B Densitometric analysis of the immunoreactive bands corresponding to p-eIF2α, ATF4, CHOP, PARP-CL, p-PERK, p-AKT in the experimental conditions shown in A, normalized for total protein content and reported as % of the control condition (WT). Data are represented as mean ± SEM. Statistical analysis was performed on three independent experiments and significance calculated by one-way ANOVA and Tukey's multiple comparisons test (°°°°p = 0.0001, °°°p = 0.0003, °°p = 0.003 for WT vs R321X; **p = 0.003, ***p = 0.0003, ****p = 0.0001 for untreated R321X vs treated R321X)