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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X

Fig. 2

Effect of salubrinal and guanabenz on cell viability and eIF2α phosphorylation. A MTT assay performed on LMNA R321X-cardiomyocytes upon 48 h incubation with salubrinal and guanabenz at three different concentrations. Statistical analysis was performed on three independent experiments and significance calculated by one-way Anova. Significancy of untreated cardiomyocytes (CTR) vs treated cardiomyocytes is reported (****p = 0.0001). B Representative Western blotting of phospho-eIF2α (p-eIF2α) performed on lysates from LMNA R321X-cardiomyocytes treated with either salubrinal or guanabenz at three different concentrations for 48 h. The triplicate of each experimental condition in the representative western blotting corresponds to three different biological replicas. C Densitometric analysis of the immunoreactive bands corresponding to p-eIF2α in the experimental conditions shown in A, normalized for total protein content and reported as % of the control condition. Data are represented as mean ± SEM. Statistical analysis was performed on three independent experiments and significance calculated by one-way ANOVA and Tukey's multiple comparisons test. Significancy of untreated cardiomyocytes (CTR) vs treated cardiomyocytes is reported (*p = 0.01, **p = 0.003, ****p = 0.0001)

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