Fig. 9

LIFR knockdown mitigates the hypertrophic effect of OSMR deficiency after AB surgery. A Schematic diagram outlining the intramyocardial injection experiment. Adenovirus-mediated short hairpin RNA (shRNA) transfer was performed through direct intramyocardial injection in the left ventricular free wall. B Representative gross morphology and haematoxylin and eosin-stained heart sections of mice subjected to AB surgery (n = 8 mice per experimental group). C Heart weight to body weight (HW/BW) ratios, lung weight to body weight (LW/BW) ratios, cross-sectional areas and echocardiographic parameters (LVEDd, LVEF, FS) of mice subjected to AB surgery (n = 8 mice/100 + cells per experimental group). D RT‒PCR analysis of ANP, BNP, and β-MHC mRNA expression in murine hearts (n = 6 per experimental group). mRNA expression was quantified and normalized to GAPDH (fold change). E Representative blots and quantitative results for OSM, LIFR, phospho-STAT3 (P-STAT3) and total STAT3 protein expression in murine hearts (n = 6 per experimental group). Data are presented as the mean ± SEM. *P < 0.05 compared with WT + AdGFP. #P < 0.05 compared with KO + AdGFP