Fig. 7

Adoptive transfer of OSMR-KO macrophages into WT mice aggravates pressure overload-induced cardiac remodelling and cardiac dysfunction. A Schematic diagram outlining the adoptive transfer experiment. PBS or BMDMs were obtained from WT or OSMR-KO mice and adoptively transferred via the tail vein into WT mice 3 days after AB surgery. B Representative gross morphology and haematoxylin and eosin-stained heart sections of mice subjected to AB surgery and adoptive transfer (n = 8 mice per experimental group). C Heart weight to body weight (HW/BW) ratios, lung weight to body weight (LW/BW) ratios, cross-sectional areas and echocardiographic parameters (LVEF) of mice subjected to AB surgery and adoptive transfer (n = 8 mice/100 + cells per experimental group). D RT‒PCR analysis of ANP, BNP, and β-MHC mRNA expression in murine hearts (n = 6 per experimental group). mRNA expression was quantified and normalized to GAPDH (fold change). E Representative blots and quantitative results for OSM, LIFR, phospho-STAT3 (P-STAT3) and total STAT3 protein expression in murine hearts (n = 6 per experimental group). Data are presented as the mean ± SEM. *P < 0.05 compared with PBS to WT. #P < 0.05 compared with WT to WT