Fig. 6

OSMR deficiency mediates inflammation and macrophage polarization in vitro. A Immunofluorescence images of F4/80 (red) and LIFR (green) in WT and KO bone marrow-derived macrophages (BMDMs) treated with LPS (scale bar = 100 μm for 400 × magnification. B Representative blots and quantitative results for OSM, LIFR, phospho-STAT3 (P-STAT3) and total STAT3 protein expression in BMDMs treated with LPS (n = 6 per experimental group). C RT‒PCR analysis of M1-like (IL-6, IL-1β, iNOS, LFA-1α) and M2-like (Arg1, IL-10, CD206, CD163) macrophage marker mRNA expression in BMDMs treated with LPS (n = 6 per experimental group). mRNA expression was quantified and normalized to GAPDH (fold change). Data are presented as the mean ± SEM. *P < 0.05 compared with WT MΦ/PBS. #P < 0.05 compared with WT MΦ/LPS