Skip to main content
Fig. 3 | Journal of Translational Medicine

Fig. 3

From: OSMR deficiency aggravates pressure overload-induced cardiac hypertrophy by modulating macrophages and OSM/LIFR/STAT3 signalling

Fig. 3

OSMR deficiency activates STAT3 signalling associated with OSM/LIFR in pressure-overloaded hearts. A RT‒PCR analysis of OSM, LIFR, IL-31, IL-31RA and LIF mRNA expression in mouse hearts at 4 weeks after sham or AB surgery (n = 6 per experimental group). mRNA expression was quantified and normalized to GAPDH (fold change). B Immunofluorescence images of OSM in mouse hearts in each group at 4 weeks after sham or AB surgery (scale bar = 100 μm for 200 × magnification). C Immunofluorescence images of LIFR in mouse hearts in each group at 4 weeks after sham or AB surgery (scale bar = 100 μm for 400 × magnification). D Representative blots and quantitative results for OSM, LIFR, phospho-STAT3 (P-STAT3) and total STAT3 protein expression in the myocardium in each group at 4 weeks after sham or AB surgery (n = 6 per experimental group). Data are presented as the mean ± SEM. *P < 0.05 compared with WT/Sham. #P < 0.05 compared with WT/AB

Back to article page