Fig. 10

Gating and quantification of immune cells in the heart and blood of mice that received intramyocardial transfer of adenovirus. A Gating strategy for flow cytometric analysis of the leukocyte population isolated from the blood of WT and OSMR-KO mice that received intramyocardial transfer of AdGFP and AdLIFRshRNA after AB surgery. Flow cytometry-based quantification of the percentage of Ly6C^high or Ly6C^low cells relative to the total monocyte/macrophage (Mo/MΦ) population and the percentage of F4/80⁺Ly6C^high cells relative to the total Mo/MΦ population. (n = 4 per experimental group). B Gating strategy for flow cytometric analysis of the leukocyte population isolated from the hearts of WT and OSMR-KO mice that received intramyocardial transfer of AdGFP and AdLIFRshRNA after AB surgery. Flow cytometry-based quantification of the percentage of Ly6C^high or Ly6C^low cells relative to the total Mo/MΦ population. (n = 4 per experimental group). Data are presented as the mean ± SEM