Fig. 6

NF1 stabilized YAP1 and hindered its ubiquitination. A The mRNA level of YAP1 was stably expressed in the NOZ cell line with knockdown of NF1. B The protein level of YAP1 was reduced in the NOZ cell line with knockdown of NF1. C CHX chase assay was performed in the lv-Con and lv-shNF1 groups. D The YAP1 protein level upon NF1 knockdown by IHC staining. E Cells were left untreated or treated with MG-132, and western blots were performed to examine the indicated protein levels. F The YAP1 underwent stronger ubiquitination in the lv-shNF1 group, which was more apparent in the cells pretreated with MG132 (10 μM, 4 h)