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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: The oncogenic role of NF1 in gallbladder cancer through regulation of YAP1 stability by direct interaction with YAP1

Fig. 5

NF1 interacted with YAP1 in NOZ cells. A Top panel, fragments of recombinant plasmids, including YAP1-FALG and NF1-MYC. The amino-acid residue numbers were indicated for each protein. Bottom panel, co-immunoprecipitation results of YAP1 and different segments of NF1. The interacting regions between NF1 and YAP1 were mapped using Co-IP assays. B Co-immunoprecipitation of YAP1 and NF12560−2818. C Co-immunoprecipitation of YAP1 and NF1AAAA. NF1AAAA represented the alanine substitution of the PPQY motif in NF12560−2818. D The protein expression levels of YAP1 in HELA and GBC cell lines. Bottom numbers: the protein levels of YAP1 relative to β-actin were quantified. E The IHC staining scores of nuclear YAP1 were higher in GBC tissues than in adjacent non-tumor tissues. ***P < 0.001. F Correlation analysis between NF1 and YAP1 expression in IHC samples. G and H Left panel, representative immunofluorescence images of YAP1 (red) and NF1 (green) in situ confirmed colocalization in NOZ cells (G) and EH-GB1 cells (H). Scale bar, 7.5 μm. Right panel, plots of the red and green pixel intensities along the white arrow in the merge panel

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