Fig. 2

Knockdown of NF1 led to impairment of proliferation and migration of GBC in vitro. A and B The mRNA and protein expression levels of NF1 in HEK 293 T, HELA, and GBC cell lines (The mRNA expression level in HELA cell was regarded as control). C and D Knockdown of NF1 in NOZ cell line was confirmed at the mRNA and protein level by qRT-PCR and western blotting. *** P < 0.001. E and F CCK-8 and colony formation assays were applied to determine the proliferation of NOZ after NF1 depletion. G and H Would healing assay and transwell assays were conducted to measure the migration of NOZ after NF1 depletion