Fig. 1

NF1 was among the top mutated genes and correlated with poor prognosis in gallbladder cancer. A NF1 was identified as one of the top 20 muted genes in gallbladder carcinoma from the COSMIC database. B The somatic mutations of NF1 in GBC were presented from the cBioPortal database, and the domain organization of NF1 was shown: NTD (N-terminal domain, dark teal), GRD (GAP-related domain, dark blue), Sec14-PH (secretory protein 14-pleckstrin homology-like module, raspberry), CDM (central dimerization module, teal), CTD (C-terminal domain, light blue). C Left panel: representative images of NF1 expression in GBC tissues and adjacent non-tumor tissues using IHC staining. Right panel: The IHC staining scores of NF1 were higher in GBC tissues than in non-tumor tissues. ***P < 0.001, n = 79. D NF1 differently expressed were subjected to further multivariate Cox regression analysis to evaluate correlation with overall survival of GBC patients (P = 0.032, n = 64)