Fig. 3

SFN-F-EXOs limit AngII-induced hypertrophy in murine HL-1 cardiomyocytes. HL-1 cardiomyocytes were seeded and treated with or without 100 nM AngII for 12 h. A F-EXOs (75 μg/mL) or SFN-F-EXOs (75 μg/mL) were added to each well, and cells were incubated for further 12 h. PBS alone was added as a control. A–B To measure cell size, HL-1 cell monolayer was fixed, permeabilized and incubated with Phalloidin-Atto550 to fluorescently stain cytoskeletal fibers. Pictures were acquired by fluorescent microscopy (20X magnification); representative pictures are shown (A). Cell area was manually measured using ImageJ. Data represent mean cell area ± SEM of three independent experiments, each one analyzed on at least five not-overlapping fields (B). *p < 0.05, ***p < 0.001, ****p < 0.0001 C, D BNP (C) and SERCA2a (D) levels were quantified by q-RT-PCR; β-actin was employed as housekeeping gene. Expression of the molecular transcript was calculated with the ΔΔCt method and shown as mean 2-ΔΔCt ± SEM of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001