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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Cardiomyocyte-targeting exosomes from sulforaphane-treated fibroblasts affords cardioprotection in infarcted rats

Fig. 2

Exosomes secretion from NIH/3t3 fibroblasts is increased by SFN treatment. NIH/3t3 cells were treated with ± 3 μM SFN in exosome-free medium for 7 days in 100 mm cell-culture dishes, medium was collected at day 3, 6 and 7. Exosomes from untreated and SFN-treated (SFN 3 μM) fibroblasts were isolated from the conditioned medium by serial centrifugation and ultracentrifugation. A Nanoparticle size distribution and concentration in the controls (NT) and SFN 3 μM pellets were measured by NTA (Nanosight technology). A representative graph of particle distribution is shown. Median particle size and particle number displayed in the bar charts represent the mean ± SEM of three independent experiments, each one analyzed in three acquisitions of one minute each. *p < 0.05. B The expression levels of exosome markers (Tsg101, HSP70 and CD81) were measured on the particles’ extracts by Western blotting. A representative blot for each protein and Ponceau S staining are shown. Densitometric data were normalized over Ponceau staining, expressed as relative to the control group (Not treated, 0 μM SFN) and represent the mean ± SEM of three independent experiments. *p < 0.01. C The expression of HSP70 was measured on total cell lysates by Western blotting. A representative blot is shown. Densitometric measurements were normalized over for the loading control (β-tubulin), expressed as relative to the control group (Not treated, 0 μM SFN) and represent the mean ± SEM of three independent experiments, each one performed in triplicate replicates. ** p < 0.01

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