Fig. 1

Identification and characterization of tumor microenvironment in non-small cell lung cancer. a Schematic representation of the experimental design and analytical methods used in this study. b Selection of the region of interest (ROI). IM, invasive margin; TC, tumor center. Representative composite images displaying multiplex staining of Panel 1 (c) and Panel 2 (d) after multispectral imaging. Each of the single-marker component image in the composite image after spectral unmixing was displayed in the enlarged subsection