Fig. 7
FOSB knockdown suppresses radioresistance of BRCA cells. A, RT-qPCR and WB analyses for mRNA (A) and protein (B) levels of FOSB in MDA-MB-231 and SK-BR-3 cells (R and Pa) after further sh-FOSB or oe-FOSB transfection; C sensitivity of MDA-MB-231 and SK-BR-3 cells (R and Pa) to different doses of irradiation ( 0, 2, 4, 8, and 12 Gy) analyzed by the CTG kit; D number of colonies formed by R or Pa BRCA cells under 8 Gy irradiation determined by colony formation assay; E, apoptosis of cells analyzed by flow cytometry; F number of apoptotic bodies formed by cells analyzed by TUNEL assay; G, H, RT-qPCR and WB assays for the mRNA (G) and protein (H) expression of γH2AX in cells. Data are expressed as the mean ± SD. Three repetitions were performed. Differences were analyzed by two-way ANOVA. **p < 0.01, ***p < 0.001