Fig. 3

Knockdown of UBE3C reduces radioresistance of BRCA cells in vivo. A, B R cells transfected with sh-UBE3C and Pa cells transfected with oe-UBE3C were injected into mice subcutaneously, followed by 16 Gy irradiation once every 3 d. Then, the volume of tissues was measured every 5 d (A); the tumors were collected and weighed on day 35 after animal euthanasia (B); C–E staining intensity of KI67 (C) PCNA (D) and γH2AX (E) in xenograft tumor tissues analyzed by IHC; F R and Pa cells were further transfected with luciferase and injected into mice intracardiacally, followed by 16 Gy irradiation once every 3 d as well. Later, the dissemination of tumor cells was analyzed by in-vivo imaging analysis once every 5 d for a total of 30 d; G number of metastatic nodules in mouse lung tissues; H survival analysis of mice in each group within 16 weeks (Kaplan-Meier analysis). In each group, n = 6 in each group. Data are expressed as the box-and-whisker plots. Differences were analyzed by one- or two-way ANOVA followed by Tukey’s multiple test. **p < 0.01, ***p < 0.001