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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: LINC00963-FOSB-mediated transcription activation of UBE3C enhances radioresistance of breast cancer cells by inducing ubiquitination-dependent protein degradation of TP73

Fig. 2

UBE3C knockdown enhances radiosensitivity of BRCA cells. A, RT-qPCR and WB analyses for mRNA (A) and protein (B) levels of UBE3C in MDA-MB-231 and SK-BR-3 cells (R and Pa) after sh-UBE3C or oe-UBE3C transfection; C sensitivity of MDA-MB-231 and SK-BR-3 cells (R and Pa) to different doses of irradiation ( 0, 2, 4, 8, and 12 Gy) analyzed by the CTG kit; D, number of colonies formed by R or Pa BRCA cells under 8 Gy irradiation determined by colony formation assay; E apoptosis of cells analyzed by flow cytometry; F, number of apoptotic bodies formed by cells analyzed by TUNEL assay; G, H RT-qPCR and WB assays for the mRNA (G) and protein (H) expression of γH2AX in cells. Data are expressed as the mean ± SD. Three repetitions were performed. Differences were analyzed by two-way ANOVA. **p < 0.01, ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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