Fig. 1

Development of spatial transcriptomics. The timeline indicates technologies (in bold blue), the years (in bold black) when the corresponding technologies were published and the journals (in dark red) where the corresponding technologies were published or employed (as in 10 × Genomics Visium). It should be noticed that scRNA-seq is presented in the figure only for reference, albeit a non-spatial technology. LCM Laser Capture Microdissection, smFISH Single-molecule RNA Fluorescence In Situ Hybridization, ISS In Situ Sequencing, TIVA Transcriptome In Vivo Analysis, FISSEQ Fluorescent In Situ RNA Sequencing, seqFISH Sequential Fluorescence In Situ Hybridization, tomo-seq RNA Tomography, MERFISH Multiplexed Error-robust Fluorescence In Situ Hybridization, smHCR Single-molecule Hybridization Chain Reaction, Geo-seq Geographical Position Sequencing, BaristaSeq Barcode In Situ Targeted Sequencing, STARmap Spatially-resolved Transcript Amplicon Readout Mapping, osmFISH Ouroboros Single-molecule RNA Fluorescence In Situ Hybridization, DSP Digital Spatial Profiling, HDST High-Definition Spatial Transcriptomics, DBiT Deterministic Barcoding in Tissue, ExSeq Expansion Sequencing, Stereo-seq Spatial Enhanced Resolution Omics-sequencing, Ex-ST Expansion Spatial Transcriptomics, PNAS Proceedings of the National Academy of Sciences of the United States of America, Nat. MethodsNature Methods, Nat. Protoc. Nature Protocols, Nucleic Acids Res. Nucleic Acids Research, Clin. Cancer Res. Clinical Cancer Research, Nat. Neurosci. Nature Neuroscience, Nat. Biotechnol. Nature Biotechnology, Sci. Adv. Science Advances