Fig. 7

Inhibitor of FSCN1 reverses the function in SYTL2-overexpressing cells. A The levels of SYTL2 and FSCN1 protein were detected by Western blotting in SYTL2-overexpressing cells combined with FSCN1 siRNA. B, C Representative images of cell migration and invasion (B) were analyzed using SYTL2-overexpressing or control cells transfected with FSCN1 siRNA, and a quantification analysis of the migrated or invaded cell counts (C) is shown. D Representative images of wound-healing assays using SYTL2-overexpressing or control cells combined with FSCN1 siRNA transfected in PC-3 cells, showing reversing cell motility after using FSCN1 siRNA in SYTL2-overexpressing cells (left panel) and a quantification analysis of the cell migration index is shown (right panel). E Representative images of three-dimensional (3D) cell culture using SYTL2-overexpressing or control cells combined with FSCN1 siRNA transfection showing reversed cell motility after using FSCN1 siRNA in SYTL2-overexpressing cells; *p < 0.05, **p < 0.01 and ***p < 0.001. Scale bars: 10 μm (red)