Fig. 4

SYTL2 regulates the protein level of FSCN1 by interacting with it. A The PCa transcriptome sequence data of TCGA were divided into two groups based on the median expression level of SYTL2 for the bubble plot of nonredundant enrichment clusters of KEGG using GSEA. B Representative image of silver-stained SDS‒PAGE gels showing separated proteins that were pulled down using Flag-labeled SYTL2. Anti-IgG was used as the negative control. C The mass spectrum of a representative peptide fragment of FSCN1. D Western blot analysis determined that SYTL2 interacts with FSCN1 after performing the pull-down assay with Flag-labeled SYTL2 (left panel) and an anti-FSCN1 (right panel) immunoprecipitation antibody. Anti-IgG was used as the negative control protein in the pull-down assay