Fig. 3

SYTL2 facilitates lymph node metastasis of PCa cells in vivo. A Representative images of the nude BALB/c mouse model of popliteal LN metastasis. The indicated PC-3 cells were injected into the footpads of the nude mice, and the popliteal LNs were enucleated and analyzed. B Representative images of bioluminescence in popliteal LN metastasis in the indicated cell groups (n = 5 per group). C–E Representative images of dissected popliteal LNs (C) and quantification analysis of the LN volume (D) and LN weight (E). F Representative images of H&E staining confirming the LN status (n = 5). The black arrow shows the PCa cell. *p < 0.05, **p < 0.01 and ***p < 0.001. Scale bars: 50 μm (red), 500 μm (black)