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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Infantile hemangioma models: is the needle in a haystack?

Fig. 7

A 3D model of the hydrogel. A. After the human IH tissue was cut into small pieces, HemECs were isolated by flow cytometry, and a three-dimensional hydrogel scaffold with ECM was prepared at the same time. Finally, HemECs were implanted into the three-dimensional scaffold for culture, and the hydrogel 3D IH model was constructed. B 3D microtumor model. Primary IH endothelial cells were isolated and cultured, and CD31+ HemECs were obtained by flow cytometry. At the same time, DAM was obtained by using tissue engineering to DAM, and DAM solution was prepared to provide specific ECM for cell culture. Then, PDMS chips with different diameters are obtained by etching characteristic patterns on silicon wafers by laser. Finally, CD31+ HemECs were plated on micropattern array petri dishes to construct 3D microtumor models

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