Fig. 5

Sp1 promoted mitochondrial morphology remodelling. A–D The proportion of globe and tube mitochondria of cervical cancer cells with treatment as indicated, detected by MicroP (Original magnification: 63×). E Mitochondrial network in cervical cancer cells treated as indicated. Arrows indicate intermediate (mid) and fragmented mitochondria. F Sp1-binding sites on the Mfn1/2, Drp1, and Opa1 promoters and G the Sp1 consensus sequence via the UCSC genome browser. H–M The levels of Mfn1/2, Drp1, and Opa1 proteins in cervical cancer cells with Sp1 knockout or a specific inhibitor (mithramycin) and overexpression, as determined by Western blotting. All experiments were repeated triplicate individually.*P < 0.05, **P < 0.01, ***P < 0.001, N. S P > 0.05