Fig. 5

The knockdown of CEBPG inhibited OC progression partially by reducing SLC7A11 expression and activating ferroptosis. A The lipid peroxidation of CEBPG-knockdown A2780 cells with or without reexpression of SLC7A11 was assessed by flow cytometry after DCFH-DA staining. B The viability of CEBPG-knockdown A2780 cells with or without SLC7A11 reexpression was assessed after treatment with Era alone or with Era plus Ferr-1. C Representative phase contrast images of CEBPG-knockdown A2780 cells treated with Era alone or combined with Ferr-1 when SLC7A11 was reexpressed. Scale bar: 100 μm. D Representative images of SLC7A11 staining in benign ovarian cyst and OC tissues. Scale bars, 200 μm and 20 μm. E Quantification of SLC7A11 expression in different tissues by IHC analysis. F Heatmap of CEBPG and SLC7A11 expression as determined by IHC analysis in different tissues. G The correlation of SLC7A11 expression levels with CEBPG levels was analyzed in a TAM. *P < 0.05, ****P < 0.0001, ns: no significance