Fig. 4
From: CEBPG suppresses ferroptosis through transcriptional control of SLC7A11 in ovarian cancer
Knockdown of CEBPG inhibited the transcription of SLC7A11 in OC cells. A The genomic distribution of CUT&Tag peaks in A2780 cells. B and C The distribution of mapped SLC7A11 peak locations within 3 kb upstream and downstream of TSSs in genes with SLC7A11-bound promoters. D The results of CUT&Tag-seq showed the binding site domain for CEBPG in the SLC7A11 gene promoter region in A2780 cells. E SLC7A11 protein expression levels in different cell lines were determined by immunoblotting. F The JASPAR website was used to scan the CEBPG-bound promoter and predict one potential binding site (GAATTTGCATCATCA) for CEBPG. The relative score (RS) = 0.890 for the motifs. G Dual luciferase reporter assay with the promoter of SLC7A11 (PLG-SLC7A11), the mutated promoter of SLC7A11 (PLG-SLC7A11 Mut) or the control promoter of SLC7A11 (PLG) in CEBPG-overexpressing (CEBPG-OE) A2780 cells. *P < 0.05, ns: no significance