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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: CEBPG suppresses ferroptosis through transcriptional control of SLC7A11 in ovarian cancer

Fig. 3

In OC, CEBPG plays a crucial role in regulating ferroptosis. A Gene expression analysis of A2780-CEBPG-Sh1 and A2780-CEBPG-NC cells. The signature was defined by the genes whose expression had changed significantly. B A TEM analysis of CEBPG-Sh1 A2780 cells was carried out after they had been treated with erastin (Era). Scale bars: 500 μm and 200 μm. C Lipid peroxidation was assessed by flow cytometry after DCFH-DA staining in CEBPG-knockdown and CEBPG-NC A2780 cells. D Cell viability was measured after treatment with Era alone, Era plus Ferr-1, Era plus Z-VAD, Era plus 3-MA or Era plus TETD in CEBPG-knockdown and CEBPG-NC A2780 cells. E Representative phase contrast images of CEBPG-knockdown A2780 cells treated with Era alone, Era plus Ferr-1, Era plus Z-VAD, Era plus 3-MA or Era plus TETD. Scale bar: 100 μm. F–H qRT‒PCR analysis of VDAC3, GPX4 and SLC7A11 expression in CEBPG-knockdown A2780 and Hey cells I CEBPG-knockdown A2780 cells with or without CEBPG reexpression were assessed for cell viability using Era alone or Era plus Ferr-1. *P < 0.05, **P < 0.01, ****P < 0.0001

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